Extraction of DNA from Human Remains.

Extraction and Analysis of Ancient DNA from Human Remains of Masjede Kabood Burial Site

An Efficient Method For DNA Extraction From Paraffin Wax Embedded Tissues For PCR Amplification Of Human And Viral DNA

  Background and Objective: Formalin-fixed paraffin-embedded tissues are a valuable source of DNA for molecular studies. We designed and optimized an efficient procedure for DNA extraction from formalin-fixed paraffin embedded tissues. Materials and Methods: Seventy three blocks of cervical paraffin-embedded tissues were investigated. DNA was extracted using 45 minutes boiling in alkaline sol...

Rapid DNA Extraction Protocol from Stool, Suitable for Molecular Genetic Diagnosis of Colon Cancer

Colorectal cancer (CRC) is one of the most common forms of cancers in the world and is curable if diagnosed at the early stage. Analysis of DNA extracted from stool specimens is a recent advantage to cancer diagnostics. Many protocols have been recommended for DNA extraction from stool, and almost allof them are difficult and time consuming, dealing with high amount of toxic materials like phen...

Ancient DNA from Human and Animal

Research on ancient DNA (aDNA) has the potential to enable molecular biologists and archeologists to decipher certain aspects of history by direct looking into the past. However, several major problems in this field limit the applicability of aDNA studies, most importantly contamination with modern DNA and postmortem DNA degradation. In this study we extracted and analyzed aDNA obtained from ~3...

ارزیابی روش های مختلف استخراج DNA از کیست ژیاردیا لامبلیا

Background and purpose: Giardia lamblia (G.lamblia) is one of flagellated protozoan pathogens. Giardiasis is the most common human parasite infection in the world, causing a lengthy course of diarrhea. The aim of the present study was to compare five different methods of DNA extraction from G.Lamblia cyst and determining the most efficient DNA extraction method. Materials and methods: This stu...

Comparison study of three methods for genomic DNA extraction from fresh and herbarium leaf specimens of Achillea wilhelmsii C.Koch

DNA extraction from plant tissues often causes most problems. For example, unsuccessful removal secondary metabolites during extraction, such as phenolic compounds in aromatic and medicinal plants, cause to some mistakes in result of molecular experiments by using of the extracted DNA. Achillea wilhelmsii is a medicinal plant belong to Asteraceae family and native to Iran, there is little infor...